ibclogo XVI International Botanical Congess

Abstract Number: 4267
Poster No. = 1956


INSERTIONAL MUTAGENESIS TO GENERATE CHEMOTACTIC MUTANTS IN CHLAMYDOMONAS REINHARDTII


E.V. Ermiova1, Zh.M. Zalutskaya1, B.V.Gromov1 , D.-P. Haeder2 & S. Purton3, 1St. Petersburg University, St. Petersburg, Russia, 2Erlangen-Nurenberg University, Erlangen, Germany, 3University College London, London, U.K.


Chlamydomonas reinhardtii, a green unicellular alga, is attracted to some sugars. Arg7-8cw15 strain was transformed with pARG7.8 linearised plasmid using glass-bead method. Of 8630 Arg+ transformants used for the selection procedure, five showed chemotactic defects. CTX2 and CTX3 did not react chemotactically to xylose. CTX1 mutant had normal taxis only to sucrose. CTX4 and CTX5 lacked chemotactic responses to all sugars. Tetrad analysis of five transformants indicated that each was due to a single nuclear mutation. Use of the ARG7 gene as an insertional mutagen has thus permitted the generation of novel chemotactic mutants which were missing with UV and MNNG.

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