XVI International Botanical Congess
A four-year continuous cultivation of Vicia faba was conducted respectively in cadmium- and lead-polluted soil and reference soil (as control). Seed was respectively obtained from polluted site and control site within every generation.The background value,dose-responses to Cd2+ and NaN3 in the four generations of Vicia faba from polluted site and control site was determined in term of Micronucleus Frequency (MCN). The results represented that, in general, with the generations having experienced more years of growing in polluted sites, the higher back ground MCN was observed, and the lower slope value in their dose-response regression equations as to laboratory treatment of cadmium ions and NaN3 solution were observed. It may be concluded that Vicia MCN test will be appropriate to be used as endpoint of biomarker in biomonitoring and bioassay of pollutants only if the test sentinel plants were collected from where no any toxic chemicals had been exposed.