ibclogo XVI International Botanical Congess

Abstract Number: 4183
Poster No. = 2212


NON-S-RNASE FACTORS INVOLVED IN POLLEN REJECTION


F. Cruz-Garcia, T. Campbell and B. A. McClure, Department of Biochemistry, University of Missouri, 117 Schweitzer Hall, Columbia, Missouri 65211, USA


Our goal is to identify and clone non-S-RNase factors required for pollen rejection. We prepared SC10-RNase affinity columns and found that several proteins in crude style extracts were bound specifically. One is recognized by anti TTS antibody. Another is a protein of 11 kD seems to form a complex with SC10-RNase and the TTS-like protein. We are also using. Using cDNA-AFLP to search for other putative factors in a population segregating for ability to reject SC10-pollen.

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