XVI International Botanical Congess

Mushroom-producing Basidiomycotina are generally considered to be nonlyophilizable. This study investigated lyophilization as a method to maintain and preserve a number of different fungi that produce edible and medicinal mushrooms. The effect of growth medium, initial cooling rates of freezing, dehydration and dehydration, and the use of lyoprotectants were analyzed. The mycelial isolates survived lyophilization and maintained their viability. No noticeable changes in morphology or biochemistry were observed, and genetic characteristics were preserved. Lyophilization f cultures of Pleurotus spp. Simulated heavy filamentous mycelial growth and fruiting was initiated within 3-7 days.