ibclogo XVI International Botanical Congess

Abstract Number: 4140
Poster No. = 1817


CLONING AND EXPRESSION OF LIPOXYGENASE GENE FROM RIPENING FRUIT OF ACTINIDIA CHINENSIS


Kunsong Chen, Changjie Xu Shanglong Zhang, Department of Horticulture, Zhejiang Agricultural University, Hangzhou 310029, P. R. China, Gavin S. Ross, The Horticulture and Food Research Institute of New Zealand Ltd., Mt. Albert Research Center. Private Bag 92 169, Auckland, New Zealand


We cloned an 824 bp long cDNA fragment encoding part of the LOX protein from A. chinensis (pKLC1). Comparisons between the sequence of the A. chinensis LOX cDNA and others from Arabidopsis, cucumber, pea, potato, rice, soybean, tobacco and tomato indicated nucleic acid homology between 59% and 75%, and amino acid identity between 63% and 76%. Southern analysis confirmed the presence of a small gene family in A. chinensis. Northern blot analysis showed that LOX gene expression is up-regulated during ripening of this fruit. Expression was up regulated following treatment with exogenous ethylene, while 0°C treatment resulted in a transient induction of gene expression, followed by down-regulation in prolonged storage. The significance of these results is discussed, in terms of a role for LOX in ripening of kiwifruit.

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