XVI International Botanical Congess

PAGE of 32 geographic isolates indicated low levels of polymorphism. These isolates and 10 UV induced mutants with the A or a mating type alleles provided sporidia for DNA extraction. Twenty C and 20 G10-mer Operon primers were used for RAPD PCR analyses for the 32 isolates using 1.2% agarose gel electrophoresis. The isolates exhibited band polymorphism and dendrogram band clustering patterns. CHEF analysis indicated that all A types had 18 similar chromosomal bands, including 2 doublets, excepting one isolate with a single band difference. All a types had 16 chromosomal bands with a 3 band difference between the 2 types. Heterologous probe hybridization of heat shock protein hsp70 to 4 isolates indicated that all isolates bound hsp70 at their 945 kb site. The GPDG gene probe bound all 4 isolates at sites 2000 and 3100 kb in the doublet. RAPD PCR and CHEF analyses detected polymorphisms within and between A and a strains which had previously been distinguished only by their mating type alleles.