ibclogo XVI International Botanical Congess


Abstract Number: 3888
Poster No. = 2168


ANALYSIS OF TOUCH-INDUCIBLE ACC SYNTHASE ISOZYMES(ACS)


Miho TATSUKI, Hitoshi MORI, Grad. Schl of Bioagri. Sci., Nagoya Univ., Nagoya 464-8601, JAPAN


To understand the molecular regulation of ethylene production by touch stimuli, we examined the expression of ACS isogenes in tomato plant. Touch stimulation of seedlings and fruits led to a large increase in the levels of LE-ACS6 and LE-ACS1A mRNA transcripts. The expressions of LE-ACS6 and LE-ACS1A isogenes increased within 10 min after touch, and mRNAs for both genes disappeared by 2 h. Thus, their expressions are transient. Wounding of tomato leaves and fruits also gave rise to transient expressions of LE-ACS6 and LE-ACS1A. The mRNA of the LE-ACS2 gene, a wound-inducible gene, was detected after 2 h in wounded tissues, but not in touched tissues. The results clearly indicate that the two isogenes, LE-ACS6 and LE-ACS1A, sense only touch, contact or pressure, and that another isogene, LE-ACS2, requires severe cell damage for its expression.


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