ibclogo XVI International Botanical Congess


Abstract Number: 3878
Session = 3.15.3


MOLECULAR CYTOGENETICS OF REPETITIVE DNA IN BETA SPECIES


Thomas Schmidt, Christine Desel, Sybille Kubis*, John S. Heslop-Harrison*University of Kiel, Germany, *John Innes Centre, Norwich, England


Fluorescent in situ hybridization and molecular methods were used to investigate the large-scale organization of the sugar beet (Beta vulgaris) genome. Major repeat classes were physically mapped and incorporated into a plant chromosome model.Centromeric and intercalary heterochromatin is largely composed of satellite DNA. Microsatellites show dispersion with site-specific amplification or exclusion and weak interspersion along chromosomes. Retrotransposons are clustered (LINEs), uniformely distributed along chromosomes with depletion at centromeres or rRNA genes (Ty1-copia retrotransposons) or amplified in many centromeres (Ty3-gypsy retrotransposons). FISH enabled the investigation of wild beet introgressions in sugar beet.T.S. acknowledges financial support of the BMBF (BioFuture grant 11860 BFB238).


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