XVI International Botanical Congess

Foreign genes were introduced to peach (Prunus persica Batsch cv.Akatuki) via Agrobacterium-mediated transformation. Three Agrobacterium strain (LBA4404, EHA101, C58C1RifR) with the plasmid having kanamycin resistance (NPTII) gene, hygromycin resistance (HPT) gene, and beta-glucuronidase (GUS) gene were infected to the leaf segments or the immature zygotic embryos. After co-cultivation, explants were cultured in the presence of 200mg/l kanamycin or 20mg/l hygromycin. Transgenic plant was obtained from the immature embryo through the adventitious embryo formation on the cotyledons. The influence of antibiotic substances on callus formation was examined.