ibclogo XVI International Botanical Congess


Abstract Number: 3752
Poster No. = 1792


COMPARISON OF UFGT GENE/PROMOTER BETWEEN WHITE GRAPES AND THEIR MUTANTS WITH RED SKIN


S.Kobayashi, M.Ishimaru, C.Ding and H.Yakushiji, Persimmon and Grape Research Center, NIFTS, Akitsu, Hiroshima, Japan


UDP glucose:flavonoid 3-o-glucosyltransferase (UFGT) has been known to play an important role in anthocyanin biosynthesis of grape berry skin. Expression of UFGT gene was not detected in white cultivars, Italia and Muscat of Alexandria, but detected in red mutants, Ruby Okuyama and Beniareki. Since these phenotypic changes were thought to be the result of mutation in the structural gene/promoter of the UFGT or mutation of regulatory genes controlling its expression, the UFGT gene/promoter sequences were analyzed firstly. However, sequence analysis revealed that there were no differences in both structural gene and promoter sequences between white cultivars and its red skin mutants. This result suggests that mutation of regulatory genes may occurred in the red mutants.


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