ibclogo XVI International Botanical Congess

Abstract Number: 3650
Session = 7.17.3


FUNCTIONAL GENOMICS AND ION TRANSPORT:CHARACTERIZATION OF ARABIDOPSIS PLANTS CONTAINING 'KNOCKOUT' MUTATIONS IN GENES ENCODING PLASMA MEMBRANE TRANSPORTERS


Michael R. Sussman, University of Wisconsin Biotechnology Center, Madison, WI 53706, Using a PCR-based protocol (Krysan, Young, Tax and Sussman, PNAS 93:8145-8150, Hirsch, Lewis, Spalding and Sussman, Science 280:918-921)


we have combed through a population of ca. 100,000 transgenic Arabidopsis lines to isolate plants containing 'knockout' mutations in a dozen genes encoding the family of P-type plasma membrane proton pumps. Also discussed will be the phenotype resulting from disruption of genes encoding key plasma membrane potassium transporters and 'signal transducing' proteins that are thought to regulate transporter activities. Using these plants, together with electrophysiolgical techniques and other transport assays, we are testing and developing models for the true in planta function of these proteins.

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