ibclogo XVI International Botanical Congess

Abstract Number: 3614
Poster No. = 1969


TEMPERATURE-REGULATED GENE ENCODING A CHLOROPLAST OMEGA-3 DESATURASE FROM GREEN ALGAE CHLORELLA SP.


Toshiya MURANAKA, Noriko YAMAGUCHI, Tetsuya NISHIDE and Masakazu MURAKAMI, Biotechnology Laboratory, Sumitomo Chemical Co., Ltd., Takarazuka, Hyogo 665-8555 Japan


In the course of experiment to isolate microalgae with high efficient in fixing CO2 capacity, we have obtained green algae Chlorella sp. MK201. Omega-3 fatty acid desaturase is responsible for the production of alpha-linolenic acid. cDNA encoding a chloroplast omega-3 fatty acid desaturase was cloned from the Chlorella. The mRNA level of the gene was rapidly increased by treatment to low temperature. Partial genomic sequence for the desaturase gene were cloned from C. sorokiniana and C. vulgaris, which are relative to Chlorella sp. MK201. The taxonomic identities of the Chlorella sp. from the sequence is also discussed.This work was supported by the New Energy and Industrial Technology Development Organization, Japan.

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