XVI International Botanical Congess

We applied a multilocus, PCR and flourescence-based microsatellite DNA marker system developed for Brassica napus to a rapid-cycling cultivar of B. rapa that we are using in a multi-generation selection experiment. In addition to six B. napus primers that amplified target sequences in its closely related congener, cross-taxa amplification with primers designed for microsatellite loci in Arabidopsis was also successful, but to a lesser degree. We present the results of genotyping three generations of plants: 1) 150 founding parents that were hand-pollinated to produce 75 full-sib lines, 2) 5 individuals from each line grown in replicated populations pollinated by bumblebees, 3) 10 progeny arrays from generation 2. Results of a paternity analysis to quantify variation in male reproductive success are presented.