ibclogo XVI International Botanical Congess

Abstract Number: 3213
Session = 19.14.4


THE ROLE OF PLANT DIVISION KINASES IN RESPONSE TO HORMONAL SIGNALS


Dénes Dudits, T Pasternak, T Mészáros, F Ayaydin, Zs Kelemen, P Miskolczi, G V. Horváth, K Kelemen, Z Magyar, A Fehér Institute of Plant Biology, BRC, HAS, Szeged, Hungary


Growth regulators can directly influence the cell division in plants. Treatment of alfalfa cells or leaf discs with 2,4-D activates CDK genes and histone H1 phosphorylation activity in p13suc1-bound protein fraction. In alfalfa at least three CDK-related kinase complexes are involved in G2/M transition. The cdc2MsA/B, D and F kinases are differentially activated by 2,4-D. The cdc2MsF kinase is active in mitotic cells, localised in preprophase band, nuclear ring in early prophase, mitotic spindle and phragmoplasts. We demonstrated the complex formation between this mitotic kinase and the D-type cyclin (cyc Ms4). Kinetin treatment of alfalfa cells in combination with auxin increased the kinase activity. Abscisic acid reduced the histone H1 phosphorylation activity.

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