ibclogo XVI International Botanical Congess

Abstract Number: 2985
Poster No. = 1789


ENVIRONMENTAL INFLUENCE ON REPETITIVE DNA BEHAVIOUR: THE LEAD/FUNARIA H. SYSTEM -


1Bassi P., 2Basile A., 2Castaldo-Cobianchi R., 3Ferraro M., 3Nardone A.M., 3Predazzi V., (1Dept. of Plant Biol., Univ. of Rome, 2Dept. of Plant Biol., Univ. of Naples, 3Dept. of Gen. & Mol. Biol., Univ. of Rome).


Repetitive DNA seems to be susceptible to environmental stress. In the in vitro controlled system Lead/Funaria h. we observed that lead is able to induce selective amplification of G+C rich repetitive DNA sequences (Bassi et al. 1995). In this work, using X-ray TEM microanalysis, in situ hybridization and molecular characterization, we obtained data that seem to indicate that nucleus is one of the target of lead and that at least part of repetitive DNA sequences lead-susceptible might belong to rDNA. Therefore we are performing FISH using as probe different portions of rDNA of Funaria h. The same probes are tested on DNA extracted from stressed and unstressed individuals. Preliminary data will be discussed.

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