XVI International Botanical Congess
In climacteric fruits, tissues undergo ethylene-mediated development. Ethylene response is determined through a regulation of ethylene production, its perception and the activation of ethylene-regulated genes. Previously, we described the characterization of two cDNA clones, Cm-ETR1 and Cm-ERS1, encoding ethylene receptors of melon that differentially accumulate during fruit development. It is most likely that ethylene sensitivity in melon is regulated by ethylene itself and is maintained by a concerted action of different receptors. To understand how these receptors are regulated during fruit ripening and by ethylene we isolated Cm-ETR1 and Cm-ERS1 from a genomic library and fused their promoter to a GUS reporter gene. Transient expression assay is in progress.