ibclogo XVI International Botanical Congess


Abstract Number: 2634
Poster No. = 1816


ETHYLENE PROMOTES NECROTIC LESION FORMATION AND SUBSEQUENT BASIC PR GENE EXPRESSION IN TMV-INFECTED TOBACCO LEAVES


Norihiro Ohtsubo1, Ichiro Mitsuhara2,3 and Yuko Ohashi2,3.1Dept. Plant Breeding, Natl. Agric. Res. Center, Tsukuba, Japan. 2Dept. Mol. Gen, Natl. Inst. Agrobiol. Resources, Tsukuba, Japan. 3CREST, JST.


Contribution of ethylene to formation of necrotic lesions and subsequent induction of basic pathogenesis-related (PR) protein genes were tested. Induction of ACC oxidase (ACO) gene expression as well as ethylene production obviously preceded the lesion appearance and ion leakage in N-gene dependent synchronous lesion formation system in tobacco. Inhibitors of ethylene biosynthesis or action suppressed both lesion formation and basic PR gene induction. Enhanced basic PR gene induction by ACC treatment was observed in ACO-overproducing transgenic tobacco plants. These results indicate that ethylene is an indispensable factor for hypersensitive reaction (HR) and basic PR gene induction.


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