XVI International Botanical Congess

A method has been described to prepare template DNA for PCR from plant tissues rich in polysacch compounds. Using the procedure it was efficient to amplify target DNA fragments, such as ITS regions and matK gene, from the materials of partial species of Rhizophoraceae which were difficult to get PCR product using normal procedure. The method involved a dilution treatment (10-15 times with ddH2O) of the colloidal mixture in which template DNA was included, obtained using the CTAB isolation method. 5-10¦Ěl diluted solution and 50¦Ěl ddH2O were mixed completely followed by purification with the Geneclean II kit. 1¦Ěl glass milk was used in the purifying step and the final glass powder with template DNA was directly used for PCR. The PCR products obtained with this procedure have been successfully used in the direct DNA sequencing.