ibclogo XVI International Botanical Congess

Abstract Number: 2528
Poster No. = 2208


A HYPERPOLARIZATION-ACTIVATED K CHANNEL FROM NICOTIANA PANICULATA


Shigehiro YAMADA, Akiko HASHIMOTO, Nobuyuki UOZUMI1, Shigetoshi OIKI2, Toshiyuki KOMORI, Hidemasa IMASEKI, Plant Breeding and Genetics Research Lab, Japan Tobacco Inc., Iwata, Shizuoka 438-0802, 1Bioscience Center, Nagoya Univ, Nagoya 464-8601, 2Department of Physiology, Fukui Medical Univ, Fukui 910-1193


A cDNA homologous to K channel genes was isolated from N. paniculata. The corresponding gene, NpKT1, encodes a polypeptide of 824 amino acids, which contained the signature sequence (TTXTTXGYGD) conserved in the K-channel superfamily. An electrophysiological analysis using the Xenopus oocyte expression system revealed that the product was a hyperpolarization-activated potassium channel. NpKT1 mRNA was detected in every organs examined, but its level was very low in roots. However, salt-stress up-regulated the expression in roots. Funded by the Research Association for Biotechnology and the Petroleum Energy Center of Japan.

HTML-Version made 7. July 1999 by Kurt Stüber