ibclogo XVI International Botanical Congess


Abstract Number: 2507
Poster No. = 1672


IDENTIFICATION OF ACTIN-AND MICROTUBULE-ASSOCIATED PROTEIN FROM TOBACCO BY-2 CELLS


H. Igarashi, S. Sonobe, T. Shimmen (Dept. Life Sci.,Himeji Inst. Tech., Harima Science Park City, Hyogo 678-1297, Japan)


Physiological studies suggested the interaction of actin filaments and microtubules in various plant cells. Although the presence of factor(s) to connect these two cytoskeletons is expected, studies in this line is sparse. The present study aims at identification of such factor(s), using cultured cells of tobacco. From the crude extract, we obtained a polypeptide (190kDa) which bound to both actin filaments and microtubules. This polypeptide localized in spindle and phragmoplast during cell division, on the other hand it localized in nuclei during interphase.


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