XVI International Botanical Congess
The hypothesis that the southern Spanish endemic A. villosa subsp. carratracensis is of hybrid origin was proposed years ago on the basis of mainly morphological evidence, and supported by reproductive studies involving the synthesis of artificial hybrids. This hypothesis is here re-examined with the aid of two molecular markers: ITS sequences and RAPDs of total DNA. Both markers provide results that are consistent with the hypothesis but none of them is conclusive. The putative hybrid taxon shares the ITS sequence with one of the putative parents but also with another taxon from the area. Diagnostic RAPD bands for the putative parents have not been detected, but a Principal Coordinate Analysis based on 155 scored bands reveals intermediacy for the putative hybrid. All these results are illustrated and compared, and likely reasons for inconclusiveness outlined.