ibclogo XVI International Botanical Congess


Abstract Number: 2470
Poster No. = 1630


ORGANOGENESIS FROM CULTURED EXPLANTS OF LETTUCE


D. C. PAINE and D. J. BURRITT Department of Botany, University of Otago, P.O. Box 56 Dunedin, New Zealand


Lettuce is grown on all continents and is a valuable horticultural crop. Genetic engineering may be a useful tool for the improvement of lettuce, but the successful use of genetic engineering requires that plants can be regenerated from transformed cells in a reliable manner. Production of adventitious shoots of lettuce usually involves culturing cotyledon explants on media containing indoleacetic acid and Kinetin. Using this combination of growth regulators the number of shoots produced per explant varies considerably and is highly genotype dependent. We have found that using a medium containing naphthalene acetic acid and benzylaminopurine the percentage of explants producing shoots and the average number of shoots produced per explant can be considerably enhanced. The influence of genotype is also reduced.


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