ibclogo XVI International Botanical Congess

Abstract Number: 2454
Poster No. = 2157


INDUCTION OF APOPTOSIS IN TOBACCO PROTOPLASTS BY HYDROXYL RADICAL.


H.M. Chen, J. Zhou, C.H. Yan, H.Z. Zhu, R.H. Tian AND Y.R. Dai. Dept. of Biology, Tsinghua University, Beijing 100084, China,


In this report we treated the protoplasts of tobacco cells with the hydroxyl radical-generating system FeSO4/H2O2, When the concentration (¦̀mol/L) ratios of FeSO4/H2O2, were 50/20,150/60 and 500/200, DNA ladders were observed with a high rate of TUNEL positive reaction. Nucleus condensation and collapse can also be seen by DAPI staining. A typical apoptotic peak appeared in hydroxyl radical-treated protoplasts by flow cytometric assay. These results illustrated that hydroxyl radicals can induce apoptosis in protoplast of tobacco cells. Moreover there is no Caspase-3 activity that can be detected in hydroxyl radical induced apoptotic cytosol when assayed by simulated substrate Ac-DEVD-pNA. It indicated that the signal transduction of apoptosis in plant cell is quite different from that in animal cells.

HTML-Version made 7. July 1999 by Kurt Stüber