ibclogo XVI International Botanical Congess


Abstract Number: 2450
Session = 3.14.1


STRUCTURE, LOCALIZATION AND FUCTION OF A NOVEL TOMATO ARABINOGALATAN-PROTEIN (AGP)


A. M. Showalter, M. Gao, M. Chen and M. J. Kieliszewski, Ohio U., Athens, OH 45701


LeAGP-1, a novel tomato AGP, was isolated from cultured cells and verified as an AGP by precipitation with Yariv reagent and AA analysis. LeAGP-1 corresponded to clones by AA composition, AA sequence and reactivity with an antibody developed against a basic subdomain predicted by clones. LeAGP-1 immunolocalized to cell walls and intercellular spaces of cultured cells, maturing metaxylem elements, stylar transmitting tissue, and the emission region and tips of pollen tubes. To probe the function of LeAGP-1 and other AGPs, antisense LeAGP-1 plants were produced and Yariv reagent was applied to cell cultures and pollen tubes. Antisense plants showed greatly reduced vegetative growth and leaf bending, while Yariv reagent induced programmed cell death in cultured cells and inhibited pollen tube growth.


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