ibclogo XVI International Botanical Congess


Abstract Number: 2431
Poster No. = 2190


ROLE OF DPPA PROTEIN AS A MOLECULAR CHAPERONE IN THE PERIPLASM OF A PHOTOTROPH


MATSUZAKI, M., Y. KISO, I. YAMAMOTO, T. SATOH, Dept. Biol. Sci., Fac. Sci., Hiroshima Univ.,


We have identified DppA as a molecular chaperone-like protein from the periplasm of Rhodobacter sphaeroides f. sp. denitrificans. In this study we isolated the dppA gene from the genome DNA and constructed dppA-disrupted strains from the phototrophs to elucidate the role of DppA in vivo as a periplasmic molecular chaperone. Two types of strains were isolated. One produced a normal level of DMSOR and the other produced little DMSOR protein in the periplasm resulting in no growth by DMSO respiration. The latter strains complemented with DppA restored the amount of the unfolded DMSOR protein although they showed no growth by DMSO respiration.


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