ibclogo XVI International Botanical Congess

Abstract Number: 2426
Poster No. = 2200


A NEWLY ISOLATED EXTRACELLULAR Mn-SOD FROM A MOSS BARBULA UNGUICULATA HAS A PEPTIDE SEQUENCE SIMILARITY WITH GERMIN


Toshiaki YAMAHARA, Takanori SUZUKI, Katsuyuki TANAKA, Susumu TAKIO1, Toshio SATOH, Dept. Biol., Fac. Sci., Hiroshima Univ.,1Dept. Biol., Fac. Sci., Kumamoto Univ.


We newly isolated an extracellular Mn-SOD in suspension-cultured cells of a moss Barbula unguiculata. An apparent molecular mass of the protein was 120 kDa by gel filtration and that of a monomer was 22 kDa by TOF mass spectrometer. It was an Asn-linked coprotein. It had one atom of manganese per monomer. The cDNA of the Mn-SOD was isolated. The amino acid sequence deduced from the base sequence shared 49% homology with that of wheat germin gf-2.8. The protein had no oxalate oxidase activity.

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