XVI International Botanical Congess

We investigated the intraspecific cpDNA variation and phylogeographic patterns of Fagus crenata Blume (Fagaceae) using 11 populations spread across the entire distribution. We determined the nucleotide sequences of following three cpDNA regions: the intergenic spacer between the trnL (UAA) 3'exon and trnF (GAA), trnL (UAA) intron, and trnK region (including matk gene). Total sequence length was approximately 3250 bp. As a result of the alignment of the sequence data, ten nucleotide substitutions and five indels were detected among the populations of F. crenata. We could recognize eight distinct cpDNA haplotypes based on the variations. Furthermore, three major clusters were revealed in most parsimony analysis, and the populations of each cluster were geographically structured.