ibclogo XVI International Botanical Congess

Abstract Number: 2376
Poster No. = 1979


CHARACTERIZATION OF PHOSPHOGLYCERATE KINASE IN CHLAMYDOMONAS REINHARDTII


M. Kitayama1, K. Kitayama2, Y. Takahashi1, and R. K. Togasaki2 (1Dept. of Food Sciences, Ehime Women's College, Uwajima, 798-0025, Japan, 2Dept. of Biol., Indiana University, Bloomington, IN 47405 USA)


Subcellular localization of phosphoglycerate kinase (PGK) in Chlamydomonas reinhardtii was studied by immuno-electron microscopy. When antibodies raised against chloroplastic phosphoglycerate kinase (chl-PGK) was used, signals were found only in the chloroplasts especially concentrated in stroma. No signal was detected in the cytoplasm. In Southern blot analysis using chl-PGK cDNA as a probe, only one band (4.2 kb Bam HI fragment) was detected. So far only one form of PGK was observed in Chlamydomonas reinhardtii and analysis of 4.2 kb fragment is underway.

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