ibclogo XVI International Botanical Congess


Abstract Number: 2221
Poster No. = 2221


IMMUNOBLOT ANALYSIS OF PROTEINS IN DISTYLOUS TURNERA


Davood Khosravi and Joel S. Shore Dept. Biology, York University, Toronto, Canada.


In distylous species, two forms of plants occur: short- and long-styled. Morphs differ in style and stamen length and possess a self incompatibility system. A protein specific to the style of the shorts and another specific to the pollen of the shorts have been identified using IEF gels. These data suggest a role for these proteins in the distylous polymorphism. We cloned 75% of the gene corresponding to the style specific protein into an expression vector and made antibodies against this fusion protein. This antibody was used for immunobloting of style proteins separated by IEF gel and SDS-PAGE. We found that on IEF blots the antibody stains the bands originally identified, and on SDS-PAGE blots it stains a band specific to the short morph but is absent in longs. The antibody also stains a band specific to short pollen, indicating cross reactivity between style and pollen proteins.


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