XVI International Botanical Congess
We described many blue-light responses in the fern Adiantum capillus-veneris. To understand the molecular mechanisms, we cloned five cryptochrome-homologues (CRY1, 2, 3, 4,and 5) at first. To analyze the correlation between these responses and CRY genes, we investigated the expression patterns in various developmental stages and in different light conditions. As a result, CRY1, CRY2 and CRY3 were not regulated by light. CRY4 and CRY5 showed unique light-regulated expression patterns. Further analyses revealed that these genes were especially regulated by phytochrome. We are investigating intracellular localizations of CRYs using the transient expression technique. We will also present these data and discuss the function of Adiantum CRYs.