ibclogo XVI International Botanical Congess

Abstract Number: 2201
Poster No. = 1911


SHUTTLE MUTAGENESIS OF THE MOSS, PHYSCOMITRELLA PATENS


T. Nishiyama* 1,2, M. Kato 1, M. Hasebe 2, * presenting author, 1 Department of Biological Sciences, Graduate School of Science, the University of Tokyo, Japan, 2 National Institut for Basic Biology, Okazaki, Japan


Physcomitrella patens is known for the high homologous recombination rate, which is suitable for molecular analyses of gene functions. To facilitate isolation of the gene from a mutant, we developed a system of tagged mutagenesis utilizing the shuttle mutagenesis (Seifert et al. 1986). We constructed mini-transposons having the G418 resistance gene, besides the bacterial ampicillin resistance gene. About 2000 stable transformants were screened for mutants of gametophyte development, and ca. 20 strains showed abnormal development. Southern analysis showed that the majority of the transformants have more than one copy of the transgene, while some have a single insert. Molecular analyses of the obtained mutants are in progress.

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