ibclogo XVI International Botanical Congess


Abstract Number: 2182
Poster No. = 1930


Genetic variation in intergenic spacer of rDNA of Imperata cylindrica (L.) populations in Taiwan


Chang-Hung Chou1, 2, Chi-Chu Tsai2, 1 Inst. of Botany, Academia Sinica, Taipei, Taiwan, 2 Graduate Inst. of Botany, Nat. Taiwan Univ., Taipei, Taiwan


Genetic variation in IGS of rDNA repetitive sequences of Cogongrass [Imperata cylindrica (L.)] populations in Taiwan was studied by employing PCR-amplified RFLP analysis. Each IGS region of 45 individuals from 15 populations was amplified. Five IGS length variants were found at 2,730 bp, 2,830 bp, 2,930 bp, 3,030 bp and 3,130 bp, of them, 2930 bp was main type IGS length. Using 13 restriction enzymes, 283 bands were revealed, and 248 bands were polymorphic. Two major clusters were found, the Chuwei population was distinctly different from the remaining populations. The findings of the PCR-amplified RFLP analysis indicated that the IGS region of rDNA provides a good genetic marker and a potential tool for microevolutionary study.


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