XVII. C. Some Major Steps in the Development of Tetrapyrrole Analytical Techniques

1. 1945: Jope and O'Brien determined the spectral absorption and fluorescence properties of coproporphyrin (Copro)I and III.
2. 1950: Rimington and Sveinsson, developed a spectrophotometric method for the determination of uroporphyrin (Uro).
3. 1950: Koski, derived simultaneous equations for the determination of Pchl(ide) and Chl a and b by absorbance spectroscopy, in unsegregated extracts.
4. 1951: Nicolas and Rimington completed the development of a paper chromatographic technique for the separation of porphyrins with 2-8 carboxyl groups.
5. 1956: Dresel and Falk, developed a method for the separation of Uro, Copro and protoprphyrin IX (Proto), by solvent partition.
6. 1966: Seliskar developed a thin-layer chromatographic technique fo the separation of fully esterified and monocarboxylic tetrapyrroles.
7. 1975:Rebeiz et al, developed spectrofluorometric techniques for the detection and quantitative determination of Proto and Pchl(ide) a formation in greening tissues.
8. 1977:Smith and Rebeiz,, developed spectrofluorometric techniques for the detection of Mg-porphyrins in the presence of Zn-porphyrins.
9. 1979: Bazzaz and Rebeiz, developed very sensitive analytical techniques for the quantitative determination of Chls and pheophyrin and Chlides and Pheophorbides by spectrofluorometry in unsegrated mixtures.
10. 1982: Daniell and Rebeiz, described a cell-free system capable of the massive conversion of ALA to Pchlide a.
11. 1982: Daniell and Rebeiz, described a cell-free system capable of the massive conversion of ALA to Chl a in the light.
12. 1984:Rebeiz et al, described the coupling of Chl a accumulation to thylakoid assembly in vitro.
13. 1985: Tripathy and Rebeiz, Developed spectrofluorometric equations for the quantitative determination of MV and DV Mg-porphyrins and MV and DV Pchl(ides).
14. 1988: Rebeiz et al, Developed equations for the determination of precursor-product relationships, in vivo, and in vitro.
15. 1989: Wu et al, developed spectrofluorometric equations for the quantitative determination of DV and MV chlorophyll(ide) a and b .
16. 1992: Shedbalkar and Rebeiz, determined the molar exctinction coefficients of DV Chl a and b and their pheophytins.
17. 1995: Parham and Rebeiz, developed an assay for [4-vinyl] Chlide a reductase using exogenous DV Chlide a.
18. 1997: Ioannides et al, Developed spectrofluorometric equations for the quantitative determination of MV Phl(ide) b.

References

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2. Rimington, C. and Sveinsson, S. L. (1950). The spectrophotometric determination of uroporphyrin. Scand. J. Clin. Lab. Invest. 2: 209-216.
3. Koski, V. M. (1950). Chlorophyll formation in seddlings of Zea mays L. Arch. Biochem. 29: 339-343.
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